Anti-Nectin 2/CD112 Rabbit Polyclonal Antibody for WB and ELISA - Q92692

Core Product Specifications and Parameters

Biological Background: Nectin-2 Function and Localization

• Nectin-2 (also known as HVEB, CD112, PVRL2) is a cell adhesion molecule belonging to the nectin family, encoded by the NECTIN2 gene.
• Functions as a modulator of T-cell signaling: acts as a costimulator upon binding to CD226, promoting T-cell proliferation and cytokine production (including IL2, IL5, IL10, IL13, IFNG); as a coinhibitor upon binding to PVRIG, inhibiting T-cell proliferation. Related references: PMID:26755705
• Probable cell adhesion protein. Related references: PMID:9657005
• Acts as a receptor for herpes simplex virus 1 mutant Rid1, herpes simplex virus 2, and pseudorabies virus, mediating viral entry.
• Localized to the cell membrane as a single-pass type I membrane protein.
• Ubiquitously expressed across human tissues, highlighting its broad physiological significance.
• Contains multiple immunoglobulin-like domains and disulfide bonds; undergoes glycosylation and phosphorylation. Alternative splicing generates multiple isoforms.
• Keywords: cell adhesion, host-virus interaction, membrane, glycoprotein, phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 382-479 in the C-terminal region of human Nectin-2 (NP_002847.1). This region may be conserved across known isoforms derived from alternative splicing.
• For Western blot, a band at approximately 58 kDa (theoretical molecular weight ~57.7 kDa) is expected; consider using a relevant positive control such as HeLa or Jurkat cell lysates.
• For ELISA, the antibody may be suitable for detecting recombinant or endogenous Nectin-2; validate coating and blocking conditions in your specific assay setup.
• Cross-reactivity with other species has not been tested; the antibody is reported to react with human Nectin-2.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive one-stop sourcing platform for IVD raw materials. Our anti-Nectin-2 antibody supports immunology, oncology, and virology research, and we can supply validated antibody pairs, bulk ancillary reagents, and rare target raw materials to accelerate your assay development. Whether you need custom monoclonal antibodies or specialized reagents, CamelBio bridges the gap from concept to clinic.