Anti-Nectin 2/CD112 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q92692

Core Product Specifications and Parameters

Biological Background: Nectin-2 Function and Localization

• Nectin-2 (CD112, PVRL2) is a type I transmembrane glycoprotein and a member of the nectin family of cell adhesion molecules.
• Modulator of T-cell signaling with competitive costimulatory and coinhibitory roles: binding to CD226 stimulates T-cell proliferation and cytokine production (including IL2, IL5, IL10, IL13, IFNG); interaction with PVRIG inhibits T-cell proliferation. Related references: PMID:26755705
• Probable cell adhesion protein. Related references: PMID:9657005
• Functions as a host cell receptor for herpes simplex virus 1 mutant Rid1, herpes simplex virus 2, and pseudorabies virus.
• Subcellular localization: cell membrane.
• Ubiquitously expressed; contains immunoglobulin-like domains, disulfide bonds, and N-glycosylation sites; subject to alternative splicing and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen (C‑terminal region aa 410‑538) may detect full‑length and select splice variants; for Western blot, the predicted molecular weight is ~58 kDa, but glycosylation can cause shifts — consider deglycosylation if needed.
• In immunohistochemistry (paraffin), antigen retrieval and antibody titration should be optimized; validate in appropriate positive and negative control tissues.
• For ELISA, pair with a suitable capture antibody or use recombinant protein standards to establish the assay.
• Cross‑reactivity with mouse and rat indicates a conserved epitope; however, validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing bridge for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For studies in immunology, virology, and cell adhesion, CamelBio can support your Nectin‑2/CD112 project from concept to clinic with reliable raw‑material supply and technical consulting.