Anti-NECAP2 Polyclonal Antibody for WB, ELISA - Q9NVZ3

Core Product Specifications and Parameters

Biological Background: NECAP2 Function and Localization

• NECAP2 (Adaptin ear-binding coat-associated protein 2) is encoded by the NECAP2 gene and is also referred to as NECAP endocytosis-associated protein 2.
• It is a component of the endocytic machinery, participating in clathrin-mediated endocytosis.
• The protein localizes to the cytoplasmic side of clathrin-coated vesicle membranes, the cell membrane, and cytoplasmic vesicles, supporting its role in vesicular trafficking.
• Alternative splicing yields multiple isoforms of NECAP2, potentially diversifying its functional roles in endocytic processes.
• NECAP2 is a phosphoprotein, indicating that its activity or interactions may be regulated by phosphorylation.
• It is implicated in protein transport, consistent with its involvement in vesicle formation and cargo sorting during endocytosis.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 28 kDa corresponding to NECAP2 is expected; consider using lysates from human cell lines (e.g., HeLa, HEK293) that express the protein.
• Given the mouse cross-reactivity, the antibody can also be tested in mouse tissue or cell lysates, but validation in the relevant sample system is recommended.
• In ELISA applications, the antibody can be tested as a capture or detection reagent; optimization of coating concentration and blocking conditions is advised.
• The immunogen covers amino acids 194–263, a region that may be accessible; consider using the antibody for immunoprecipitation after empirical validation, though not listed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-NECAP2 reagent, and bulk ancillary reagents for endocytosis research. Whether you require rare target antibodies or large-scale production support, CamelBio bridges your needs from concept to clinic, supporting diagnostic developers and research institutions with reliable sourcing solutions.