Anti-NECAB3 Rabbit Polyclonal Antibody for WB and ELISA - Q96P71

Core Product Specifications and Parameters

Biological Background: NECAB3 Function and Localization

• Alternative names include NIP1, XB51, STIP3, EFCBP3, SYTIP2, and APBA2BP; belongs to the N-terminal EF-hand calcium-binding protein family.
• Inhibits the association between APBA2 and the amyloid-beta precursor protein (APP), thereby promoting amyloid-beta generation, a key process in Alzheimer's disease pathogenesis.
• Under normoxic conditions, may enhance HIF1A transcriptional activity to drive glycolysis, an effect requiring its ABM domain and potentially involving stabilization of the HIF1AN–APBA3 complex.
• Localizes predominantly to the Golgi apparatus, where it may participate in calcium-dependent signaling or protein trafficking.
• Strongly expressed in heart and skeletal muscle, with moderate levels in brain and pancreas, indicating tissue-specific regulatory roles.
• Predicted molecular weight of approximately 44 kDa; undergoes alternative splicing and is identified as a phosphoprotein in proteomic studies.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–362, encompassing the N‑terminal EF‑hand domains; consider this region’s conservation when assessing cross‑reactivity and epitope accessibility.
• For Western blot, the expected band size is around 44 kDa. Use positive control lysates from human, mouse, or rat heart or skeletal muscle, where NECAB3 is highly expressed.
• In ELISA applications, optimize coating conditions and antibody concentrations via checkerboard titration; the recombinant immunogen may serve as an appropriate coating antigen.
• Since the antibody recognizes human, mouse, and rat NECAB3, it can support cross‑species studies; validate reactivity in your specific model system before large‑scale experiments.
• Store aliquots at −20 °C or −80 °C and avoid repeated freeze–thaw cycles to preserve antibody integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with a comprehensive catalog of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti‑NECAB3 polyclonal antibody, relevant to Alzheimer’s disease and calcium signaling research, exemplifies our commitment to supplying hard‑to‑find targets. Let our end‑to‑end sourcing services accelerate your assay development from concept to the clinic.