Anti-NECAB1 Rabbit Polyclonal Antibody for WB, ELISA - Q8N987

Core Product Specifications and Parameters

Biological Background: NECAB1 Function and Localization

• NECAB1 (N-terminal EF-hand calcium-binding protein 1), also known as neuronal calcium-binding protein 1, is encoded by the NECAB1 gene (alternative names: EFCAB1, EFCBP1).
• It belongs to the family of EF-hand calcium-binding proteins, featuring conserved EF-hand motifs that coordinate calcium ions, implicating it in calcium signaling pathways.
• The protein contains a coiled-coil domain, which may mediate protein-protein interactions.
• NECAB1 is predominantly expressed in the brain as evidenced by protein-level detection, suggesting a specialized role in neuronal function.
• Subcellularly, it localizes to the cytoplasm.
• Post-translational modifications include phosphorylation, and the protein is reported to exist in multiple isoforms due to alternative splicing.
• Keywords associated with NECAB1 include metal-binding, calcium, and repeat domains, highlighting its structural and regulatory capacity.

Experimental Guidance and Technical Tips

• The immunogen covers the majority of the NECAB1 sequence (amino acids 1–351), potentially enabling recognition of full-length and alternatively spliced isoforms.
• For western blot, brain tissue lysates may serve as a suitable positive control given the brain-specific expression of NECAB1.
• When using cross-species applications, validate reactivity in mouse and rat samples, as cross-reactivity is indicated but may require optimization.
• As a polyclonal antibody, lot-to-lot consistency should be verified, especially for quantitative assays like ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for diagnostic manufacturers and research institutions, offering comprehensive access to IVD raw materials and technical support from concept to clinic. Our portfolio includes validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents, with this anti-NECAB1 antibody serving as a reliable reagent for neuroscience research applications. We also support rare target sourcing and bulk supply needs, helping you streamline your assay development and production workflows.