Anti-NDUFV2 Polyclonal Antibody for WB, ELISA - P19404

Core Product Specifications and Parameters

Biological Background: NDUFV2 Function and Localization

• NDUFV2 (NADH dehydrogenase [ubiquinone] flavoprotein 2), also known as CI-24k, is a nuclear-encoded mitochondrial protein that serves as a core subunit of respiratory chain Complex I.
• It resides in the peripheral arm of Complex I, where it accepts electrons from NADH via flavin mononucleotide (FMN) and transfers them along a chain of iron-sulfur clusters to ubiquinone, facilitating proton pumping across the inner mitochondrial membrane.
• The protein contains one 2Fe-2S iron-sulfur cluster that is essential for its electron transfer activity.
• NDUFV2 is localized to the mitochondrial inner membrane.
• Mutations in the NDUFV2 gene are linked to primary mitochondrial disease and Complex I deficiency (MC1DN7), underscoring its critical role in cellular energy metabolism.
• Post-translational modifications, including acetylation and phosphorylation, have been identified, potentially modulating its function or assembly.
• The protein is highly conserved among mammals (human, mouse, rat), consistent with the observed cross-reactivity of this antibody.

Experimental Guidance and Technical Tips

• For Western blot (WB) analysis, the expected band size for NDUFV2 is approximately 27 kDa; consider using mitochondrial-enriched lysates or whole-cell extracts from relevant tissues to enrich target detection.
• In ELISA applications, this polyclonal antibody can be used for detecting recombinant or endogenous NDUFV2; consider pairing with a validated detection antibody for sandwich ELISA development.
• Given the high sequence conservation among human, mouse, and rat, cross-reactivity should be validated in the specific sample system of interest.
• Optimization of antibody dilution and incubation conditions is recommended based on the experimental model and detection method.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, providing diagnostic manufacturers and research laboratories with a comprehensive range of high-quality antibodies, antigen pairs, and ancillary reagents. In the field of mitochondrial research and metabolic disorders, we offer validated polyclonal and monoclonal antibodies, bulk ancillary reagents, and support for rare-target raw material procurement. Whether you are developing immunoassays for Complex I subunits or require custom antibody generation, we streamline your supply chain from concept to clinic.