Anti-NDUFV1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P49821

Core Product Specifications and Parameters

Biological Background: NDUFV1 Function and Localization

• NDUFV1 encodes the NADH dehydrogenase [ubiquinone] flavoprotein 1, also known as Complex I-51kD (CI-51kD), a core subunit of mitochondrial respiratory chain Complex I.
• As part of the peripheral arm of Complex I, NDUFV1 contains flavin mononucleotide (FMN) which serves as the initial electron acceptor from NADH, and an iron-sulfur cluster (4Fe-4S) that facilitates electron transfer to ubiquinone via a chain of iron-sulfur clusters (PMID:28844695). Related references: PMID:28844695
• NDUFV1 is localized to the mitochondrial inner membrane, where it participates in electron transport and proton translocation, coupling to ATP synthesis.
• The protein belongs to the NADH-ubiquinone oxidoreductase flavoprotein family and is highly conserved across species, with orthologs in mouse and rat.
• Alternative splicing of the NDUFV1 gene produces multiple transcript variants, potentially yielding isoforms with distinct N-terminal sequences; the immunogen corresponds to the N-terminal 250 amino acids of the canonical isoform.
• NDUFV1 undergoes post-translational modifications including acetylation and methylation, and it contains a transit peptide for mitochondrial import.
• Mutations in NDUFV1 are associated with Leigh syndrome, a severe mitochondrial disease characterized by progressive neurological degradation.
• Keywords for NDUFV1 include: Flavoprotein, FMN, 4Fe-4S, Iron, Metal-binding, NAD, Oxidoreductase, Respiratory chain, Ubiquinone, Mitochondrion inner membrane, and Primary mitochondrial disease.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 1-250 of human NDUFV1; this N-terminal region may detect full-length protein and potentially some splice variants.
• For western blot (WB), the predicted molecular weight is ~51 kDa; consider using mitochondrial-rich tissue or cell lysates (e.g., heart, muscle, or cultured cells) to ensure sufficient antigen abundance.
• In IHC-paraffin (IHC-P) and immunofluorescence/ICC (IF/ICC), pre-validate antibody dilution and signal specificity with appropriate positive and negative control tissues/cells, given the mitochondrial localization.
• The antibody cross-reacts with mouse and rat NDUFV1, indicating recognition of a conserved epitope; this may be advantageous for studies in rodent models.
• For ELISA applications, pairing with a suitable detection antibody may allow measurement of NDUFV1 levels in mitochondrial extracts or purified Complex I.
• As with all polyclonal antibodies, lot-to-lot variability can occur; consider validating in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a seamless one-stop sourcing experience for IVD raw materials, from validated antibody pairs to rare target antibodies like this NDUFV1 polyclonal antibody. Whether you need bulk ancillary reagents for mitochondrial respiratory chain assays or custom development support for Complex I-related diagnostics, our end-to-end services—from concept to clinic—accelerate your project timelines. Contact us to discuss how we can tailor NDUFV1 antibody supply to your specific requirements.