Anti-NDUFV1 Monoclonal Antibody for WB, IHC-P, ELISA - P49821

Core Product Specifications and Parameters

Biological Background: NDUFV1 Function and Localization

• NDUFV1 encodes the NADH dehydrogenase [ubiquinone] flavoprotein 1, a core subunit of mitochondrial complex I (NADH-ubiquinone oxidoreductase), also known as Complex I-51kD or CI-51kD.
• It functions as the primary electron acceptor in the peripheral arm of complex I, binding FMN and a 4Fe-4S cluster to initiate electron transfer from NADH to ubiquinone, driving proton translocation across the inner mitochondrial membrane. Related references: PMID:28844695
• The protein is located in the mitochondrial inner membrane, with a processed mature form of approximately 51 kDa after cleavage of the mitochondrial transit peptide.
• NDUFV1 belongs to the respiratory chain complex I subunit family, and mutations in this gene are associated with Leigh syndrome and other primary mitochondrial disorders.
• Post-translational modifications include acetylation and methylation, and the protein contains essential metal-binding motifs for iron-sulfur clusters.
• Alternative splicing and proteomics identification support a single major isoform in human tissues, with predominant expression in high-energy-demand organs.

Experimental Guidance and Technical Tips

• The immunogen (aa 72-420) covers a large portion of the mature protein, ensuring robust detection of total NDUFV1 in WB applications; consider running a positive control such as mitochondrial lysates from human, mouse, or rat tissues.
• For IHC-P, antigen retrieval conditions may need optimization depending on tissue type; antibody specificity can be validated using siRNA knockdown or complex I inhibitor-treated samples.
• In ELISA, the antibody may be suitable for both direct and indirect detection formats—validate in the relevant sample matrix to confirm sensitivity and linearity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including validated antibody pairs, recombinant proteins, and bulk ancillary reagents. For mitochondrial and metabolic disease research, we offer a range of optimized monoclonal and polyclonal antibodies against complex I subunits like NDUFV1, enabling reliable detection across multiple applications. Whether you need custom-formulated immunoreagents or rare-target sourcing for clinical assay development, CamelBio bridges the gap from concept to clinic with expert technical support and global supply capabilities.