Anti-NDUFS8 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O00217

Core Product Specifications and Parameters

Biological Background: NDUFS8 Function and Localization

• NDUFS8 encodes the NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, a core subunit of mitochondrial Complex I (also known as CI-23kD or TYKY subunit) essential for electron transfer from NADH to ubiquinone. Related references: PMID:22499348
• It is critical for the catalytic activity and assembly of Complex I, facilitating proton translocation across the inner mitochondrial membrane. Related references: PMID:22499348
• Localizes exclusively to the mitochondrial inner membrane, as indicated by its subcellular location.
• Expressed in all tissues, with the highest levels in heart and skeletal muscle and the lowest level in lung.
• Contains iron-sulfur clusters (4Fe-4S) and participates in redox reactions as an oxidoreductase, utilizing NAD and ubiquinone.
• Genetic variants in NDUFS8 are associated with primary mitochondrial diseases, including complex I deficiency (MC1DN2), highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 35-210 of human NDUFS8, which may recognize both the human and mouse orthologs (cross-reactivity validated for mouse).
• For Western blotting, a band near 24 kDa is expected; consider mitochondrial enrichment for optimal detection in tissue lysates.
• In IF/ICC, confirm mitochondrial localization by co-staining with organelle markers and use appropriate fixation/permeabilization protocols.
• When applying ELISA, optimize the coating antigen and antibody dilution using the recombinant immunogen as a reference standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio enables diagnostic manufacturers and research institutions to streamline IVD raw material procurement, from concept to clinic. This anti-NDUFS8 antibody supports investigation into mitochondrial Complex I function and related disorders, and CamelBio can additionally supply matched antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for large-scale assay development. Whether you need rare target sourcing or comprehensive technical support, CamelBio is your trusted partner in mitochondrial research tools.