Anti-NDUFS7 Rabbit Polyclonal Antibody for WB, ELISA - O75251

Core Product Specifications and Parameters

Biological Background: NDUFS7 Function and Localization

• NDUFS7 encodes the NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, a core subunit of mitochondrial respiratory chain Complex I, also known as CI-20kD or PSST subunit.
• It is essential for Complex I catalytic activity, where it mediates electron transfer from NADH to ubiquinone and contributes to proton pumping across the inner mitochondrial membrane (PubMed:17275378). Related references: PMID:17275378
• The protein contains a 4Fe-4S iron-sulfur cluster critical for its electron carrier function in the respiratory chain.
• NDUFS7 localizes to the mitochondrial inner membrane, the site of oxidative phosphorylation and ATP synthesis.
• Alternative splicing of the NDUFS7 gene produces multiple isoforms, potentially modulating complex I assembly or function.
• Post-translational modifications of NDUFS7 include hydroxylation, as identified in proteomic studies.
• Pathogenic variants in NDUFS7 are causally linked to Leigh syndrome, a severe form of mitochondrial disease, underscoring its clinical significance.

Experimental Guidance and Technical Tips

• For Western blot (WB) detection, consider using mitochondrial-enriched protein lysates from human cells or tissues to improve sensitivity and specificity for NDUFS7.
• The predicted molecular weight of NDUFS7 is 22–23 kDa; however, actual migration may vary depending on gel and sample conditions; validate with a positive control.
• For ELISA applications, the synthetic peptide immunogen (amino acids 80–160) can be used as a coating antigen to assess antibody binding.
• As a polyclonal antibody, it may recognize multiple isoforms of NDUFS7; confirm target specificity by knockdown or knockout models if necessary.
• Cross-reactivity is reported for human; if using other species, verify sequence homology of the immunogen region before experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide a comprehensive portfolio of IVD raw materials. This anti-NDUFS7 rabbit polyclonal antibody is part of our mitochondrial research collection, supporting complex I and respiratory chain studies. Beyond antibodies, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets—streamlining your path from discovery to diagnostic application.