Anti-NDUFS6 Polyclonal Antibody for WB, ELISA - O75380

Core Product Specifications and Parameters

Biological Background: NDUFS6 Function and Localization

• NDUFS6, also known as Complex I-13kD-A (CI-13kD-A), is a nuclear-encoded subunit of the mitochondrial NADH dehydrogenase complex (Complex I).
• It functions as an accessory subunit and is not directly involved in the catalytic mechanism of electron transfer from NADH to ubiquinone.
• Localized to the mitochondrial inner membrane, NDUFS6 is an integral component of the respiratory chain.
• The protein is subject to acetylation and contains a transit peptide for mitochondrial import, as indicated by its UniProt keywords.
• Disease-associated variants in NDUFS6 have been linked to primary mitochondrial disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 29-124 of human NDUFS6 (NP_004544.1), covering the mature protein region. Consider this when planning epitope mapping or competition assays.
• For Western blotting, the predicted molecular weight is approximately 14 kDa; include appropriate molecular weight markers and validate the band pattern in your sample type.
• Cross-reactivity with mouse and rat is reported; however, verify antibody specificity and expression levels in your experimental system.
• ELISA applications may require optimization of coating antigen and antibody titration; consider using the recombinant immunogen as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing platform for IVD raw materials, supporting your NDUFS6 and mitochondrial research needs. From validated antibody pairs to bulk ancillary reagents, we connect diagnostic manufacturers and labs with high-quality reagents and technical resources to streamline development from concept to clinic.