Anti-NDUFS5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O43920

Core Product Specifications and Parameters

Biological Background: NDUFS5 Function and Localization

• NDUFS5 encodes the NADH dehydrogenase [ubiquinone] iron-sulfur protein 5, also known as Complex I-15 kDa (CI-15 kDa) subunit.
• It functions as an accessory subunit of mitochondrial respiratory chain Complex I, without a direct catalytic role, and participates in electron transfer from NADH to ubiquinone.
• NDUFS5 is part of the NDUFS (NADH:ubiquinone oxidoreductase iron-sulfur) protein family, contributing to the assembly and stability of Complex I.
• This protein localizes to the mitochondrial inner membrane and intermembrane space, consistent with its role in the electron transport chain.
• UniProt annotates NDUFS5 with keywords such as Disulfide bond, Electron transport, and Respiratory chain, indicating structural and functional features.
• The calculated molecular weight is ~12.5 kDa (UniProt), consistent with the observed ~13 kDa band.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment covering amino acids 1-106 of human NDUFS5. This N-terminal region may be conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot (WB), the predicted band is ~13 kDa; ensure appropriate gel percentage and transfer conditions for low-molecular-weight proteins.
• For immunofluorescence (IF/ICC), MitoTracker co-staining may be used to confirm mitochondrial localization.
• For ELISA, the antibody can be used to capture or detect NDUFS5 in cell or tissue lysates; optimization of coating and detection conditions is recommended.
• As with any polyclonal antibody, lot-to-lot consistency may vary; validation in the relevant sample system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with consolidated access to premium IVD raw materials. This anti-NDUFS5 antibody is part of our portfolio targeting mitochondrial respiratory chain components, alongside validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents. For rare targets like Complex I subunits, CamelBio leverages deep supplier networks to streamline procurement from concept to clinic.