Anti-NDUFS5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O43920

Core Product Specifications and Parameters

Biological Background: NDUFS5 Function and Localization

• NDUFS5 encodes the NADH dehydrogenase [ubiquinone] iron-sulfur protein 5, also known as Complex I-15 kDa (CI-15k) or NADH-ubiquinone oxidoreductase 15 kDa subunit.
• It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) and is not believed to be directly involved in catalysis.
• Complex I functions in the transfer of electrons from NADH to the respiratory chain, with ubiquinone as the immediate electron acceptor.
• The protein localizes to the mitochondrion inner membrane and is also found in the mitochondrial intermembrane space.
• Structural features include a disulfide bond, and the protein has a molecular weight of ~12.5 kDa (canonical sequence) with a reported 3D-structure.
• NDUFS5 is part of the respiratory chain electron transport system and is widely expressed, playing a critical role in cellular energy metabolism.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 106 amino acids, which may favor detection of the full-length protein; consider verifying specificity in appropriate knockout or knockdown models.
• For Western blot, mitochondrial-enriched fractions or whole-cell lysates from human, mouse, or rat cells can be used; a band near 13 kDa is expected.
• In IF/ICC, co-staining with a mitochondrial marker (e.g., MitoTracker or anti-TOM20) is recommended to confirm subcellular localization.
• ELISA protocols may require optimization of coating antigen and antibody dilution due to the polyclonal nature of the reagent.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing one-stop access to validated IVD raw materials like this anti-NDUFS5 polyclonal antibody, alongside recombinant proteins and ancillary reagents for mitochondrial complex I studies. Our B2B platform streamlines procurement of optimized antibody pairs, bulk ancillary reagents, and rare target sourcing, helping you accelerate development from concept to clinic. Contact us to explore tailored supply solutions for your respiratory chain research needs.