Anti-NDUFS4 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O43181

Core Product Specifications and Parameters

Biological Background: NDUFS4 Function and Localization

• NDUFS4 is an accessory subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase), not directly involved in catalysis but essential for complex stability and assembly.
• It mediates electron transfer from NADH to ubiquinone within the respiratory chain, contributing to mitochondrial ATP production.
• The protein is localized to the mitochondrial inner membrane, anchoring Complex I to the membrane.
• Alias names include CI-18 kDa, CI-AQDQ, and AQDQ, reflecting its historical designation as an 18 kDa subunit of Complex I.
• Post-translational modifications include phosphorylation, which may regulate Complex I activity.
• Mutations in NDUFS4 are associated with mitochondrial complex I deficiency nuclear type 1 (MC1DN1), a severe neurodegenerative disorder.

Experimental Guidance and Technical Tips

• The antibody was raised against the N-terminal region (aa 1-175) of human NDUFS4, a sequence highly conserved across human, mouse, and rat, supporting cross-reactivity.
• For western blot, expect a band near 20 kDa. Optimize blocking and washing conditions for mitochondrial membrane proteins.
• When performing IHC-P or IF/ICC, include a permeabilization step to access the mitochondrial inner membrane target.
• Validate the antibody in cell lines or tissues with high Complex I expression, such as heart, skeletal muscle, or brain.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized polyclonal/monoclonal antibodies, and bulk ancillary reagents to support your mitochondrial and respiratory chain research. Our supply capabilities encompass rare target sourcing and flexible production scales, directly connecting diagnostic manufacturers with critical reagents for every stage from concept to clinic.