Anti-NDUFS4 Rabbit Monoclonal Antibody for WB, ELISA - O43181

Core Product Specifications and Parameters

Biological Background: NDUFS4 Function and Localization

• NDUFS4 encodes the NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, a 20 kDa accessory subunit of mitochondrial Complex I (NADH-ubiquinone oxidoreductase). Alternative names include Complex I-18 kDa (CI-18 kDa), Complex I-AQDQ (CI-AQDQ), and AQDQ.
• It is not directly involved in catalysis but is essential for the structural integrity and assembly of Complex I, which transfers electrons from NADH to ubiquinone in the respiratory chain.
• Located on the mitochondrial inner membrane, with a transit peptide directing its import.
• The protein is subject to phosphorylation, as indicated by its inclusion in the phosphoprotein keyword, potentially modulating Complex I activity or stability.
• Mutations in NDUFS4 are associated with primary mitochondrial disease, including Leigh syndrome (MC1DN1), highlighting its clinical relevance.
• Additional Uniprot keywords: 3D-structure, Disease variant, Electron transport, Membrane, Mitochondrion, Mitochondrion inner membrane, Phosphoprotein, Primary mitochondrial disease, Proteomics identification, Reference proteome, Respiratory chain, Transit peptide, Transport.

Experimental Guidance and Technical Tips

• The immunogen region (aa 43–175) is conserved across human, mouse, and rat, supporting the observed cross-reactivity. Consider sequence alignment before extending to other species.
• For Western blot, expect a band around 20 kDa. Use mitochondrial-enriched protein extracts and validate with appropriate loading controls.
• This antibody is suitable for ELISA. Optimization of coating antigen concentration and antibody dilution is recommended.
• Validate performance in tissues with high mitochondrial content (e.g., heart, skeletal muscle) to ensure sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This NDUFS4 antibody supports research into mitochondrial disorders and respiratory chain deficiencies. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic development.