Anti-NDUFS4 Monoclonal Antibody for WB - O43181

Core Product Specifications and Parameters

Biological Background: NDUFS4 Function and Localization

• NDUFS4 encodes the NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, an accessory subunit of mitochondrial Complex I. It is also known as Complex I-18 kDa (CI-18 kDa) and Complex I-AQDQ (CI-AQDQ).
• Complex I mediates the transfer of electrons from NADH to ubiquinone in the respiratory chain; NDUFS4 is not catalytic but is essential for complex assembly or stability.
• The protein localizes to the mitochondrial inner membrane, directed by an N-terminal transit peptide.
• NDUFS4 is a phosphoprotein, suggesting post-translational regulation of its function.
• Mutations in NDUFS4 are associated with primary mitochondrial diseases, highlighting its critical role in cellular energy metabolism.
• The mature human NDUFS4 protein has a molecular weight of approximately 20 kDa.
• A three-dimensional structure of NDUFS4 has been resolved, providing insights into its integration within Complex I.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 1-100 of human NDUFS4, which includes the mitochondrial targeting sequence; for detection of the mature protein, a band of ~20 kDa is expected in western blot.
• For IHC-P, consider optimizing antigen retrieval; for IF/ICC, confirm mitochondrial localization using counterstains.
• In ELISA applications, titrate the antibody to determine optimal working concentration for your assay format.
• Validate cross-reactivity in mouse and rat samples, as protein sequence homology in the immunogen region may affect binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-NDUFS4 antibody supports mitochondrial research and diagnostic development. We also assist in rare target raw-material sourcing, providing one-stop access from concept to clinic.