Anti-NDUFS3 Rabbit Polyclonal Antibody for WB, IP, ELISA - O75489

Core Product Specifications and Parameters

Biological Background: NDUFS3 Function and Localization

• NDUFS3 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), catalyzing electron transfer from NADH to ubiquinone and essential for complex I catalytic activity and assembly. Related references: PMID:14729820 PMID:30140060 PMID:24028823
• Subcellular localization: Mitochondrion inner membrane.
• Encoded by the NDUFS3 gene; mutations are linked to mitochondrial complex I deficiency (MC1DN8) and primary mitochondrial disease.
• Alternative splicing produces multiple isoforms; the protein undergoes cleavage of a transit peptide upon mitochondrial import.
• Molecular weight approximately 30 kDa; belongs to the complex I 30 kDa subunit family.
• Keywords: 3D-structure, alternative splicing, disease variant, electron transport, membrane, mitochondrion, NAD, oxidoreductase, primary mitochondrial disease, respiratory chain, translocase, ubiquinone.

Experimental Guidance and Technical Tips

• For WB detection, consider enriching mitochondrial fractions to enhance signal, as NDUFS3 localizes to the inner membrane.
• Validate antibody performance in human, mouse, and rat samples, as cross-reactivity is reported.
• In IP experiments, the polyclonal nature may co-immunoprecipitate Complex I components; appropriate controls are recommended.
• For ELISA, optimize coating antigen concentration and detection conditions for the specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing platform for IVD raw materials, technical services, and consulting from concept to clinic. This anti-NDUFS3 rabbit polyclonal antibody is part of our mitochondrial research portfolio, providing reliable reagents for respiratory chain studies. We supply validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to streamline your diagnostic development.