Anti-NDUFS3 Rabbit Monoclonal Antibody (Complex I-30kD) for WB, IHC-P, ELISA - O75489

Core Product Specifications and Parameters

Biological Background: NDUFS3 Function and Localization

• NDUFS3, also known as Complex I-30kD (CI-30kD) or NADH-ubiquinone oxidoreductase 30 kDa subunit, is encoded by the NDUFS3 gene and belongs to the mitochondrial complex I iron-sulfur protein family. Alternative splicing is observed, contributing to protein diversity.
• It functions as a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), catalyzing electron transfer from NADH to ubiquinone, a process essential for cellular respiration. Related references: PMID:14729820, PMID:30140060
• NDUFS3 is indispensable for the catalytic activity and proper assembly of Complex I. Related references: PMID:14729820, PMID:24028823, PMID:30140060
• The protein localizes to the mitochondrion inner membrane, where it participates in the electron transport chain.
• Keywords associated with NDUFS3 include electron transport, respiratory chain, oxidoreductase, NAD, ubiquinone, transit peptide, translocase, and primary mitochondrial disease. Disease variants have been reported, linking mutations in NDUFS3 to mitochondrial disorders.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was generated against a recombinant fusion protein corresponding to amino acids 37-264 of human NDUFS3 (NP_004542.1). The immunogen design targets a central region of the protein, which may be suitable for detecting both full-length and certain isoforms.
• Validated applications include Western blot (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), and ELISA. For WB, a band at ~30 kDa is expected. Consider using appropriate controls and titrating antibody concentrations.
• Cross-reactivity is reported for human, mouse, and rat. When working with other species, sequence homology should be verified prior to experimental use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized procurement for rare targets. For mitochondrial research and respiratory chain disorders, this anti-NDUFS3 monoclonal antibody supports applications from basic biology to diagnostic development. Our streamlined supply chain bridges concept to clinic, enabling researchers and manufacturers to accelerate timelines with reliable, high-quality reagents.