Anti-NDUFS3 Monoclonal Antibody for WB, IHC-P, ELISA - O75489

Core Product Specifications and Parameters

Biological Background: NDUFS3 Function and Localization

• NDUFS3 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) and is essential for its catalytic activity and assembly, catalyzing electron transfer from NADH to ubiquinone. Related references: PMID:14729820 PMID:24028823 PMID:30140060
• It is also known as Complex I-30kD (CI-30kD) or NADH-ubiquinone oxidoreductase 30 kDa subunit.
• NDUFS3 localizes to the mitochondrial inner membrane.
• Mutations in this gene cause mitochondrial complex I deficiency (MC1DN8), a primary mitochondrial disease.
• Alternative splicing results in multiple transcript variants.
• The mature protein has a calculated molecular weight of about 30.2 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 165-264 of human NDUFS3. For Western blotting, expect a band near 30 kDa; mitochondrial-enriched lysates or complex I preparations may enhance detection.
• For immunohistochemistry (IHC-P), consider antigen retrieval methods and validate on tissues with high mitochondrial content, such as heart or skeletal muscle.
• In ELISA, the peptide immunogen corresponds to an internal sequence; use recombinant NDUFS3 protein or cell lysates as coating antigens, and confirm specificity with appropriate controls.
• Cross-reactivity is confirmed for human and rat; for other species, verify sequence homology before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for diagnostic manufacturers and research laboratories, providing comprehensive IVD raw materials for mitochondrial and respiratory chain studies. Beyond this NDUFS3 antibody, we supply validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents to accelerate your assay development. Our team also assists in sourcing rare targets and custom raw materials, supporting your projects from concept to clinic.