Anti-NDUFS2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O75306

Core Product Specifications and Parameters

Biological Background: NDUFS2 Function and Localization

• NDUFS2 is a core subunit of mitochondrial complex I, catalyzing electron transfer from NADH to ubiquinone and essential for respiratory chain activity. Related references: PMID:22036843, PMID:28031252, PMID:30922174

• The protein is critical for complex I assembly and catalytic function.

• NDUFS2 plays a key role in acute oxygen sensing, mediating hypoxic pulmonary vasoconstriction and carotid body hypoxia responses. Related references: PMID:30922174

• It localizes to the mitochondrial inner membrane.

• The protein contains a 4Fe-4S cluster and undergoes modifications such as acetylation and methylation.

• Alternative splicing produces multiple isoforms.

• Mutations in NDUFS2 are linked to Leber hereditary optic neuropathy and other primary mitochondrial diseases.

• NDUFS2 is involved in neural stem/progenitor cell proliferation and differentiation (By similarity).

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight of NDUFS2 is 53 kDa; consider using mitochondrial-enriched lysates to enhance signal.
• The immunogen (amino acids 204-463) is within the mature protein; validate detection of endogenous NDUFS2 in human, mouse, or rat tissue extracts.
• For immunohistochemistry (IHC-P), optimization of antigen retrieval and antibody dilution may be required.
• This antibody has been KO validated; use parental or wild-type cells as a positive control and NDUFS2 knockout cells as a negative control to confirm specificity.
• For immunofluorescence (IF/ICC), permeabilization and appropriate fixation methods should be tested.
• In ELISA, the recombinant antibody can be used for antigen capture or detection; perform a checkerboard titration to determine optimal concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined, end-to-end solution for IVD raw material sourcing, from initial antibody screening to bulk reagent supply. Our portfolio includes validated polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents essential for mitochondrial complex I research and diagnostic development. Leverage our expertise to source high-quality NDUFS2 antibodies and related tools for your next assay development project.