Anti-NDUFS1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P28331

Core Product Specifications and Parameters

Biological Background: NDUFS1 Function and Localization

• NDUFS1 encodes the NADH-ubiquinone oxidoreductase 75 kDa subunit, also known as Complex I-75kD (CI-75kD), a core component of mitochondrial respiratory chain complex I.
• The protein contains 2Fe-2S and 4Fe-4S iron-sulfur clusters that are critical for electron transfer from NADH to ubiquinone, a key step in cellular respiration.
• NDUFS1 plays a key role in the entry and efficient transfer of electrons within complex I, and is essential for complex I assembly, stability, and the formation of supercomplexes with complex III. Related references: PMID:30879903, PMID:31557978
• The protein is anchored in the mitochondrial inner membrane and is ubiquitously expressed, reflecting its fundamental role in energy metabolism.
• Post-translational modifications, including acetylation, and alternative splicing generate multiple isoforms, potentially modulating complex I function.
• Mutations in NDUFS1 are linked to mitochondrial complex I deficiency (MC1DN5), a severe disorder affecting oxidative phosphorylation, highlighting its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 80–290 of human NDUFS1, which includes regions of the N-terminal iron-sulfur cluster domain; the polyclonal nature may recognize multiple epitopes and isoforms.
• For Western blot (WB), NDUFS1 migrates at a predicted molecular weight of ~79 kDa; verify band specificity using appropriate positive (e.g., mitochondrial lysates) and negative controls.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate the antibody in tissue or cell lines known to express NDUFS1, and consider antigen retrieval conditions for optimal signal.
• For ELISA, the polyclonal antibody can serve as a capture or detection reagent; pair with a monoclonal antibody for higher specificity if needed.
• Cross-reactivity has been demonstrated with human, mouse, and rat; however, experimental validation in your sample system is recommended to confirm reactivity and performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-NDUFS1 polyclonal antibody supports research into mitochondrial disorders and oxidative phosphorylation, and CamelBio can source validated antibody pairs, optimize monoclonal/polyclonal antibody production, and supply bulk ancillary reagents for your assay development. Whether you need rare-target raw materials or end-to-end diagnostic solutions, we bridge the gap between discovery and commercialization.