Anti-NDUFS1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P28331

Core Product Specifications and Parameters

Biological Background: NDUFS1 Function and Localization

• NDUFS1 is a core subunit of mitochondrial Complex I (NADH-ubiquinone oxidoreductase), catalyzing electron transfer from NADH to ubiquinone and essential for Complex I assembly, stability, and supercomplex formation with Complex III.
  Related references:
  PMID:30879903
  PMID:31557978
• The protein contains iron-sulfur clusters (2Fe-2S, 4Fe-4S) critical for electron transport and is post-translationally modified by acetylation. Alternative splicing produces multiple isoforms.
• Subcellular localization: Mitochondrion inner membrane.
• Mutations in NDUFS1 are linked to primary mitochondrial disease (MC1DN5), underlining its clinical relevance.
• Belongs to the NADH dehydrogenase (ubiquinone) iron-sulfur protein family; molecular weight ~79 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the NDUFS1 protein (amino acids 80–290 of NP_004997.4). For Western blot, expect a band around 79 kDa in human, mouse, and rat lysates.
• For IHC-P, consider antigen retrieval optimization as the target localizes to the mitochondrial inner membrane.
• As a polyclonal antibody, lot-to-lot variability may occur; validate performance in your specific experimental system.
• Recommended applications (WB, IHC-P, ELISA) are based on supplier data; verify optimal dilutions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined one-stop procurement platform for IVD raw materials, serving diagnostic manufacturers and research institutes worldwide. This anti-NDUFS1 polyclonal antibody supports mitochondrial Complex I research and disease model validation. Beyond individual antibodies, CamelBio offers validated antibody pairs, bulk reagent supplies, and specialized sourcing for rare targets to accelerate your assay development pipeline.