Anti-NDUFS1 Monoclonal Antibody for WB, IHC-P, ELISA - P28331

Core Product Specifications and Parameters

Biological Background: NDUFS1 Function and Localization

• NDUFS1 encodes the NADH-ubiquinone oxidoreductase 75 kDa subunit, a core component of mitochondrial respiratory chain Complex I, also known as CI-75kD.
• It catalyzes the transfer of electrons from NADH to ubiquinone, a critical step in oxidative phosphorylation (PubMed:30879903, PubMed:31557978). Related references: PMID:30879903 PMID:31557978
• Essential for the assembly and stability of Complex I and its association with Complex III to form supercomplexes (PubMed:30879903, PubMed:31557978). Related references: PMID:30879903 PMID:31557978
• Contains 2Fe-2S and 4Fe-4S iron-sulfur clusters, which are essential for its oxidoreductase activity and electron transport function.
• Localized to the mitochondrial inner membrane, consistent with its role in the electron transport chain.
• Post-translational modifications include acetylation, and the protein undergoes alternative splicing to produce multiple isoforms.
• Mutations in NDUFS1 are linked to mitochondrial complex I deficiency, nuclear type 5 (MC1DN5), a severe neuromuscular disorder.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 80-290 of human NDUFS1, a region conserved in mouse and rat, supporting cross-reactivity in these species.
• For Western blot (WB), the expected molecular weight is approximately 79 kDa; consider using a suitable molecular weight marker and loading control.
• When performing immunohistochemistry (IHC-P), antigen retrieval may be required; validate with appropriate positive and negative control tissues.
• In ELISA applications, consider pairing this antibody with a validated capture/detection system for optimal signal.
• As a recombinant rabbit monoclonal antibody, it offers high batch-to-batch consistency and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials. This NDUFS1 antibody is suitable for researchers studying mitochondrial disorders and respiratory chain function. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and custom sourcing for rare targets—streamlining your development from concept to clinic.