Anti-NDUFC2 Rabbit Monoclonal Antibody for WB, ELISA - O95298

Core Product Specifications and Parameters

Biological Background: NDUFC2 Function and Localization

• NDUFC2 encodes the NADH dehydrogenase [ubiquinone] 1 subunit C2, also designated CI‑B14.5b (Complex I‑B14.5b), HLC‑1 (Human lung cancer oncogene 1 protein), and NADH‑ubiquinone oxidoreductase subunit B14.5b.
• As an accessory subunit of mitochondrial complex I, NDUFC2 is not directly involved in catalysis but is required for the proper assembly and stability of the enzyme complex.
• Complex I catalyzes the transfer of electrons from NADH to ubiquinone, a central step of the mitochondrial respiratory chain that drives oxidative phosphorylation.
• The protein localizes to the mitochondrial inner membrane, in line with its role in the electron transport chain.
• Alternative splicing produces multiple transcript variants, implying the existence of protein isoforms that may differ in tissue distribution or functional modulation.
• Mutations in NDUFC2 are associated with primary mitochondrial diseases, reflecting its essential role in complex I integrity and cellular energy metabolism.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against an immunogen covering the N‑terminal 119 amino acids; it is expected to recognize the full‑length endogenous NDUFC2 protein (~14 kDa).
• For Western blotting, validate the antibody in human cell or tissue lysates; a single band near 14 kDa should be observed. Consider using mitochondrial‑enriched fractions to enhance signal.
• For ELISA applications, test direct or sandwich formats with recombinant NDUFC2 or native complex I as coating antigen; cross‑reactivity with other species has not been evaluated, so limit to human samples unless verified.
• As NDUFC2 is a membrane protein, ensure that lysis and sample buffers are compatible with hydrophobic proteins to avoid aggregation or loss.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti‑NDUFC2 monoclonal antibody supports mitochondrial disease research and complex I assembly investigations. Through our global supply network, we also offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare‑target raw materials to accelerate your assay development and production scaling.