Anti-NDUFC2 Polyclonal Antibody for WB, ELISA - O95298

Core Product Specifications and Parameters

Biological Background: NDUFC2 Function and Localization

• NDUFC2 (NADH dehydrogenase [ubiquinone] 1 subunit C2, alias CI-B14.5b) is an accessory subunit of mitochondrial Complex I, essential for complex assembly rather than catalysis.
• Complex I functions in the transfer of electrons from NADH to ubiquinone within the mitochondrial respiratory chain.
• The protein localizes to the mitochondrial inner membrane and contains a predicted transmembrane helix.
• Multiple isoforms are produced by alternative splicing.
• Mutations in NDUFC2 are associated with primary mitochondrial disease, specifically mitochondrial complex I deficiency nuclear type 36 (MC1DN36).
• 3D structural information is available for this subunit.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the N-terminal region (aa 1-100) of human NDUFC2; this region may be conserved across species, supporting the observed cross-reactivity with mouse and rat.
• Suitable for Western blot (WB) and ELISA. For WB, consider using appropriate mitochondrial or whole-cell lysates and loading controls (e.g., VDAC, COXIV) to verify specific detection of the ~14 kDa band.
• Due to alternative splicing, multiple isoforms may be expressed; validate banding patterns in your target sample system.
• Store at -20°C or as recommended upon receipt; shipped on ice bags to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibodies, bulk reagents, and tailored supply solutions to streamline your diagnostic and research workflows. This anti-NDUFC2 polyclonal antibody supports mitochondrial respiratory chain studies, and we can assist with ancillary reagents, optimized antibody pairs, and rare-target sourcing to accelerate your project from concept to clinic.