Anti-NDUFB9 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9Y6M9

Core Product Specifications and Parameters

Biological Background: NDUFB9 Function and Localization

• NDUFB9, also known as CI-B22 or LYRM3, is a nuclear-encoded accessory subunit of the mitochondrial NADH dehydrogenase complex (Complex I) that is not directly involved in catalysis but is essential for complex assembly or stability.
• Complex I transfers electrons from NADH to ubiquinone in the respiratory chain, coupling this process to proton translocation across the inner mitochondrial membrane; NDUFB9 assists in maintaining this electron transfer chain.
• As a member of the LYR motif-containing protein family, NDUFB9 features a conserved LYR domain that may mediate protein-protein interactions within the complex.
• The protein is anchored to the mitochondrial inner membrane, where Complex I is embedded, positioning it within the oxidative phosphorylation machinery.
• Post-translational modifications include acetylation and phosphorylation, which may regulate Complex I activity or assembly dynamics under metabolic conditions.
• Mutations in NDUFB9 have been associated with primary mitochondrial disease, specifically Complex I deficiency, underscoring its clinical relevance in metabolic disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 80-170 of human NDUFB9, a region that is likely well-conserved across species, consistent with reported cross-reactivity with mouse and rat. Consider Western blot sample preparation with mitochondrial-enriched fractions to enhance signal.
• For IHC-P, antigen retrieval methods and tissue fixation conditions may impact antibody binding; validate in both frozen and paraffin-embedded sections from relevant tissues.
• In ELISA, the antibody can be used as a capture or detection reagent; pairing with a compatible secondary antibody is recommended, and optimal dilutions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with validated raw materials for mitochondrial biology and metabolic research. We offer this anti-NDUFB9 rabbit monoclonal antibody alongside complementary reagents—including antibody pairs, bulk ancillary proteins, and custom sourcing for rare targets—to streamline your assay development pipeline.