Anti-NDUFB9 Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y6M9

Core Product Specifications and Parameters

Biological Background: NDUFB9 Function and Localization

• NDUFB9, also known as CI-B22, LYRM3, or UQOR22, is an accessory subunit of the mitochondrial respiratory chain Complex I (NADH dehydrogenase).
• It is believed not to be directly involved in catalysis but plays a structural or regulatory role in the complex.
• Complex I mediates electron transfer from NADH to ubiquinone, coupling this to proton translocation across the inner mitochondrial membrane.
• The protein is encoded by the NDUFB9 gene (GeneID: 4715) and belongs to the LYR motif-containing protein family.
• NDUFB9 localizes to the mitochondrial inner membrane, consistent with its role in oxidative phosphorylation.
• Post-translational modifications include acetylation and phosphorylation; the protein has been directly sequenced and identified via proteomics.
• Mutations in NDUFB9 are associated with mitochondrial complex I deficiency, a primary mitochondrial disease.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 80–170 of human NDUFB9; when assessing cross-reactivity with mouse and rat orthologs, consider aligning this region.
• For western blotting (WB), a band at approximately 22 kDa (calculated molecular weight 21.8 kDa) is expected.
• The antibody is applicable to IHC-P and ELISA; validate in your specific sample system (e.g., mitochondrial extracts or tissue sections) to confirm performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials. This NDUFB9 polyclonal antibody supports studies in mitochondrial disorders and oxidative phosphorylation. For bulk orders, validated antibody pairs, or ancillary reagents, CamelBio offers reliable supply and technical consultation from concept to clinic.