Anti-NDUFB8 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - O95169

Core Product Specifications and Parameters

Biological Background: NDUFB8 Function and Localization

• NDUFB8 encodes the NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, also referred to as Complex I-ASHI (CI-ASHI). It is an accessory subunit of mitochondrial respiratory chain Complex I, not directly involved in catalysis.
• As a structural component, NDUFB8 contributes to the assembly and stability of Complex I, which facilitates electron transfer from NADH to ubiquinone, a critical step in oxidative phosphorylation.
• The protein localizes to the mitochondrial inner membrane, as indicated by its transmembrane helix and transit peptide, anchoring it within the electron transport chain.
• Alternative splicing yields multiple isoforms; mutations in NDUFB8 are linked to primary mitochondrial disease, specifically mitochondrial complex I deficiency, nuclear type 32 (MC1DN32).
• It plays a role in electron transport, membrane transport, and respiratory chain function, and is annotated as a reference proteome member with resolved 3D-structure.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fusion protein spanning amino acids 1–150 of human NDUFB8, which covers the majority of the mature protein. This design may enable detection of full-length NDUFB8 (predicted ~22 kDa) in various assays.
• For Western blot, a band at approximately 22 kDa is anticipated; cross-reactivity is reported for human, mouse, and rat samples, allowing multi-species validation.
• In immunofluorescence (IF/ICC and IF-P) and immunohistochemistry (IHC-P), mitochondrial localization should be evident. Consider permeabilization protocols optimized for mitochondrial membrane antigens.
• ELISA applications may benefit from pairing this antibody with suitable capture or detection reagents; validate against recombinant NDUFB8 or native complex I extracts to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop supplier for IVD raw materials, offering this anti-NDUFB8 rabbit monoclonal antibody to support diagnostic assay development in mitochondrial disorders. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets, ensuring seamless procurement from research to clinical application.