Anti-NDUFB8 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95169

Core Product Specifications and Parameters

Biological Background: NDUFB8 Function and Localization

• NDUFB8 is an accessory subunit of mitochondrial respiratory chain Complex I and is not directly involved in catalysis.
• Complex I catalyzes the transfer of electrons from NADH to ubiquinone, playing a central role in oxidative phosphorylation.
• The protein localizes to the mitochondrial inner membrane.
• Alternative splicing generates multiple isoforms, and mutations in NDUFB8 are associated with mitochondrial complex I deficiency (MC1DN32), a primary mitochondrial disease.
• Post-translational modifications and proteomics identification have been reported, contributing to its functional regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 29-186 of human NDUFB8, excluding the mitochondrial transit peptide; consider using mitochondrial enrichment protocols for optimal detection.
• The antibody has been reported to react with human, mouse, and rat samples; cross-reactivity with other species should be validated experimentally.
• For Western blotting, a band around 22 kDa is expected; adjust gel percentages and transfer conditions accordingly.
• In immunostaining applications (IHC-P, IF/ICC), include appropriate controls to confirm mitochondrial localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, technical services, and consulting, supporting every stage from concept to clinic. This anti-NDUFB8 polyclonal antibody is part of our extensive portfolio for mitochondrial research, and we can also supply validated antibody pairs, optimized monoclonals, and bulk ancillary reagents. For custom or rare-target raw material needs, our team is ready to assist with tailored sourcing solutions.