Anti-NDUFB4 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95168

Core Product Specifications and Parameters

Biological Background: NDUFB4 Function and Localization

• NDUFB4 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which transfers electrons from NADH to ubiquinone.
• This subunit is believed not to be directly involved in catalysis but contributes to the structural integrity and assembly of Complex I.
• The protein localizes to the mitochondrial inner membrane and contains a transmembrane helix, consistent with its membrane-bound function.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics studies (keyword: Phosphoprotein, Acetylation).
• Alternative splicing generates multiple transcript variants, potentially contributing to isoform diversity (keyword: Alternative splicing).
• NDUFB4 is part of the NADH:ubiquinone oxidoreductase supernumerary subunit family, distinct from the catalytic core subunits.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–90) of human NDUFB4, which is predicted to be exposed and may facilitate recognition of the full-length protein.
• For western blotting (WB), a band around 15 kDa is expected; consider including a mitochondrial fraction or Complex I immunocapture to verify specificity.
• In immunohistochemistry (IHC-P), antigen retrieval conditions may need optimization; a granular cytoplasmic staining pattern consistent with mitochondrial localization should be observed.
• In immunofluorescence (IF/ICC), a mitochondrial staining pattern can be validated by co-staining with a mitochondrial marker (e.g., MitoTracker or anti-TOM20).
• The antibody cross-reacts with mouse and rat NDUFB4, making it suitable for studies in rodent models. Validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This anti-NDUFB4 polyclonal antibody exemplifies our commitment to supporting mitochondrial and respiratory chain research, with cross-reactivity for human, mouse, and rat models. Whatever your target—whether common or rare—our streamlined sourcing services help bridge the gap from concept to clinic.