Anti-NDUFB4 (CI-B15) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O95168

Core Product Specifications and Parameters

Biological Background: NDUFB4 Function and Localization

• NDUFB4 (also designated CI-B15 or NADH-ubiquinone oxidoreductase B15 subunit) is a 129-amino acid accessory subunit of mitochondrial respiratory chain Complex I.
• Complex I (NADH dehydrogenase) transfers electrons from NADH to ubiquinone; NDUFB4 is not directly involved in catalysis but contributes to the structural integrity of the complex.
• It is an integral membrane protein with a predicted transmembrane helix, localized to the mitochondrial inner membrane.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its function or assembly.
• The NDUFB4 gene undergoes alternative splicing, though the functional significance of potential isoforms remains unclear.
• As part of the electron transport chain, NDUFB4 and Complex I are central to cellular energy metabolism and are linked to various mitochondrial disorders.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human NDUFB4 (aa 1-129); confirm sequence conservation if using non-human samples, though the antibody cross-reacts with mouse and rat.
• For Western blotting, the expected molecular weight is approximately 15 kDa; using mitochondrial-enriched fractions or gentle lysis conditions may improve detection sensitivity.
• In IHC-P, consider antigen-retrieval protocols due to the target's membrane localization; validate in known positive tissues (e.g., heart, skeletal muscle, kidney).
• This monoclonal antibody can be employed as a capture or detection reagent in sandwich ELISA; pairing with another clone or a polyclonal antibody may yield optimal assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and laboratories, offering validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and support for rare target sourcing. This NDUFB4 antibody serves as a reliable tool for mitochondrial Complex I research and respiratory chain dysfunction diagnostics. Partner with CamelBio to access end-to-end supply solutions—from concept to clinic—for your assay development needs.