Anti-NDUFB3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O43676

Core Product Specifications and Parameters

Biological Background: NDUFB3 Function and Localization

• NDUFB3, also known as Complex I-B12 (CI-B12), is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) and is not directly involved in catalysis.
• Complex I transfers electrons from NADH to ubiquinone, a critical step in the respiratory chain; NDUFB3 is believed to contribute to the structural integrity and assembly of the complex.
• Encoded by the NDUFB3 gene, this 11 kDa protein is associated with primary mitochondrial disease (MC1DN25) when mutated, highlighting its physiological importance.
• The protein contains a transmembrane helix and is localized to the mitochondrion inner membrane.
• Post-translational modifications include acetylation and methylation, as identified through proteomics.
• Electron transport, membrane, and respiratory chain are among its functional keywords.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human NDUFB3 (amino acids 1-98), suggesting the antibody may recognize both native and denatured protein forms; consider testing in relevant mitochondrial enrichment or whole-cell lysates for western blot.
• For IF/ICC, mitochondrial localization is expected; co-staining with mitochondrial markers may help confirm specificity.
• Cross-reactivity with mouse NDUFB3 is reported; validate in mouse tissue or cell lines to ensure suitability for translational studies.
• ELISA can be used for quantification or screening; consider pairing with a detection system optimized for rabbit antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-NDUFB3 polyclonal antibody as part of a comprehensive portfolio of IVD raw materials for mitochondrial research. We support diagnostic manufacturers with validated antibody pairs, bulk reagents, and access to rare targets, simplifying your journey from concept to clinic.