Anti-NDUFB11 Polyclonal Antibody for WB, ELISA - Q9NX14

Core Product Specifications and Parameters

Biological Background: NDUFB11 Function and Localization

• NDUFB11 is an accessory subunit of mitochondrial Complex I (NADH dehydrogenase), which is not directly involved in the catalytic mechanism but contributes to complex stability and function.
• Complex I transfers electrons from NADH to ubiquinone, a key step in the mitochondrial respiratory chain for ATP production.
• Encoded by the NDUFB11 gene, the protein is also known as CI-ESSS, Np17.3, and ESSS, and plays a role in mitochondrial electron transport.
• It resides in the mitochondrial inner membrane and is ubiquitously expressed across human tissues.
• Mutations in NDUFB11 are associated with primary mitochondrial disease, including cardiomyopathy and encephalopathy, highlighting its importance in cellular energy metabolism.
• The protein undergoes alternative splicing, and its function is critical for proper respiratory chain activity.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant protein covering amino acids 1-163 of human NDUFB11; expect detection of the full-length protein at approximately 17 kDa in Western blot.
• For WB analysis, validate in the relevant sample system (human, mouse, or rat) and optimize antibody dilution to achieve specific signal.
• Since this is a polyclonal antibody, consider lot-to-lot consistency checks and always include appropriate controls.
• For ELISA applications, the antibody can be used as capture or detection reagent; pair with a matched antibody for sandwich ELISA if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including extensively validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers and diagnostic developers focusing on mitochondrial respiratory chain targets like NDUFB11, CamelBio provides one-stop procurement support from concept to clinic, ensuring reliable supply for your assay development needs.