Anti-NDUFB10 Polyclonal Antibody for WB, IP, ELISA - O96000

Core Product Specifications and Parameters

Biological Background: NDUFB10 Function and Localization

• NDUFB10 is an accessory subunit of mitochondrial complex I (NADH:ubiquinone oxidoreductase), which catalyzes the transfer of electrons from NADH to ubiquinone in the respiratory chain.
• It participates in the functional assembly of complex I, contributing to the structural integrity of the membrane arm.
• The protein is localized to the mitochondrial inner membrane.
• Alternative splicing generates multiple transcript variants, and mutations are associated with primary mitochondrial disease (MC1DN35).
• It is also known as PDSW (Complex I-PDSW) and is subject to phosphorylation.
• The subunit is part of a large multi-protein complex; high-resolution 3D structures have been determined for complex I.
• It belongs to the complex I NDUFB10 subunit family.

Experimental Guidance and Technical Tips

• The immunogen is a full-length human NDUFB10 recombinant protein (aa 1-172); ensure sequence conservation when testing in mouse or rat samples.
• For Western blot, expect a band at ~21 kDa; consider using mitochondrial-enriched lysates to enhance signal.
• In immunoprecipitation, validate antibody performance with positive controls such as isolated mitochondria or cells expressing tagged NDUFB10.
• For ELISA, consider pairing with another clone or anti-tag antibody for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including this anti-NDUFB10 polyclonal antibody, to support mitochondrial and respiratory chain disease research. We offer validated antibody pairs, bulk reagents, and assistance in sourcing specialized raw materials for diagnostic and laboratory applications. Our integrated supply chain helps you accelerate from concept to clinic.