Anti-NDUFAF2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8N183

Core Product Specifications and Parameters

Biological Background: NDUFAF2 Function and Localization

• NDUFAF2 (also known as B17.2L, mimitin, NDUFA12L) is a mitochondrial molecular chaperone essential for the assembly of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex (complex I) (PubMed:16200211, PubMed:19384974). Related references: PMID:16200211, PMID:19384974
• Complex I catalyzes the transfer of electrons from NADH to ubiquinone in the respiratory chain, a critical step for oxidative phosphorylation and cellular energy production (PubMed:16200211, PubMed:27626371). Related references: PMID:16200211, PMID:27626371
• Beyond its role in mitochondrial bioenergetics, NDUFAF2 is involved in early steps of ciliogenesis, including removal of CP110 from mother centrioles, vesicle docking, and transition zone establishment (PubMed:38949024). Related references: PMID:38949024
• The protein localizes to the mitochondria, consistent with its function in complex I assembly and energy metabolism.
• NDUFAF2 is highly expressed in ESCC (esophageal squamous cell carcinoma) cells and also found in heart, skeletal muscle, liver, and fibroblasts.
• Disease-associated variants in NDUFAF2 are linked to primary mitochondrial diseases, underscoring its clinical relevance.
• Post-translational modifications include phosphorylation, which may regulate its chaperone activity or interactions.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein spanning amino acids 1-169 of human NDUFAF2; it may detect both full-length and N-terminal fragments.
• For Western blot, the predicted molecular weight is approximately 20 kDa; validate in your sample system and consider loading mitochondria-enriched fractions to enhance signal.
• In immunofluorescence/immunocytochemistry, expect a mitochondrial localization pattern; co-staining with a mitochondrial marker is recommended for confirmation.
• The antibody is also applicable for ELISA, which can be used to quantify NDUFAF2 levels in cell lysates or purified mitochondrial preparations.
• Since the antibody cross-reacts with mouse and rat NDUFAF2, it can be used for comparative studies across these species; however, always validate in the specific tissues or cells of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This NDUFAF2 polyclonal antibody supports research in mitochondrial complex I biology, ciliogenesis, and mitochondrial diseases. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development and diagnostic pipeline.