Anti-NDUFAF1 Monoclonal Antibody for WB, IF/ICC, ELISA - Q9Y375

Core Product Specifications and Parameters

Biological Background: NDUFAF1 Function and Localization

• NDUFAF1 is also known as Complex I intermediate-associated protein 30, mitochondrial (CIA30), CGI-65, and MC1DN11.
• Encoded by the NDUFAF1 gene (GeneID: 51103), the protein has a calculated molecular weight of approximately 38 kDa (UniProt: 37764 Da) and contains a transit peptide for mitochondrial targeting.
• NDUFAF1 functions as a chaperone within the MCIA (mitochondrial complex I assembly) complex, facilitating the assembly of the multi-subunit NADH dehydrogenase (complex I) of the oxidative phosphorylation system.
• The protein localizes to the mitochondrial matrix, where it participates in the early stages of complex I biogenesis.
• NDUFAF1 is ubiquitously expressed across human tissues, consistent with its fundamental role in cellular respiration.
• Post-translational modifications include phosphorylation (as indicated by the Phosphoprotein keyword), and mutations in the NDUFAF1 gene are associated with primary mitochondrial disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-150) of human NDUFAF1. This design may contribute to specificity for the native mitochondrial protein.
• For Western blot, consider enriching mitochondrial fractions to enhance signal detection, as the target is localized to this organelle.
• Due to its ubiquitous expression, HeLa or HEK293 cell lysates may serve as suitable positive controls.
• In IF/ICC experiments, verify mitochondrial localization by co-staining with organelle-specific markers.
• For ELISA, optimizing coating conditions with recombinant NDUFAF1 protein as a standard may improve assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, supporting projects from concept to clinic. This NDUFAF1 monoclonal antibody exemplifies our offering of validated antibodies for mitochondrial research and respiratory chain disorders. For bulk orders, custom antibody development, or rare target sourcing, CamelBio serves as your reliable procurement partner.