Anti-NDUFAB1 Rabbit Polyclonal Antibody for WB, ELISA - O14561

Core Product Specifications and Parameters

Biological Background: NDUFAB1 Function and Localization

• NDUFAB1 encodes mitochondrial acyl carrier protein (ACP), also known as CI-SDAP and NADH-ubiquinone oxidoreductase 9.6 kDa subunit. It is expressed in human mitochondria (UniProt: O14561).
• As an acyl carrier protein, NDUFAB1 carries the growing fatty acid chain during mitochondrial fatty acid biosynthesis, playing a key role in lipid metabolism. Related references: PMID:27626371
• NDUFAB1 serves as an accessory subunit of mitochondrial complex I (NADH dehydrogenase), participating in electron transport from NADH to the respiratory chain. Related references: PMID:27626371
• Together with LYRM4, NDUFAB1 regulates the stability and cysteine desulfurase activity of NFS1 within the core iron-sulfur cluster (ISC) assembly complex, facilitating [2Fe-2S] cluster assembly on ISCU. Related references: PMID:31664822
• The protein undergoes post-translational modifications, including phosphopantetheine attachment and acetylation, and is localized exclusively in the mitochondrion, as indicated by its transit peptide (Keywords: Acetylation, Phosphopantetheine, Phosphoprotein, Mitochondrion).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 50–156 of human NDUFAB1, which likely avoids the mitochondrial targeting sequence; consider that this design may enable detection of the endogenous, processed form of the protein.
• For western blotting, the predicted molecular weight is 17 kDa; validate the band specificity in mitochondrial-rich samples and consider using subcellular fractionation to enrich the signal.
• For ELISA applications, consider using recombinant NDUFAB1 protein or the immunizing peptide as the coating antigen to assess antibody binding.
• Cross-reactivity is reported for human, mouse, and rat; if applying to other species, verify sequence homology in the immunogen region before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. We support diagnostic manufacturers and research labs in sourcing rare and specialized targets such as NDUFAB1, facilitating studies in mitochondrial complex I biology, fatty acid metabolism, and iron-sulfur cluster assembly. With a commitment to bridging concept to clinic, CamelBio streamlines your procurement of high-quality immunological reagents.