Anti-NDUFAB1 Rabbit Polyclonal Antibody for IF/ICC and ELISA - O14561

Core Product Specifications and Parameters

Biological Background: NDUFAB1 Function and Localization

• NDUFAB1 encodes mitochondrial acyl carrier protein, also known as CI-SDAP, NADH-ubiquinone oxidoreductase 9.6 kDa subunit, ACP, ACP1, SDAP, and FASN2A.
• It serves as the carrier of the growing fatty acid chain in mitochondrial fatty acid biosynthesis. Related references: PMID:27626371
• Acts as an accessory and non-catalytic subunit of mitochondrial Complex I (NADH dehydrogenase), mediating electron transfer from NADH to the respiratory chain. Related references: PMID:27626371
• Functions as an accessory protein of the core iron-sulfur cluster (ISC) assembly complex, regulating NFS1 cysteine desulfurase activity and participating in [2Fe-2S] cluster assembly on ISCU in association with LYRM4. Related references: PMID:31664822
• Localizes to the mitochondrion.
• Undergoes post-translational modifications including acetylation, phosphorylation, and phosphopantetheine attachment, and is involved in pathways such as electron transport, fatty acid metabolism, and respiratory chain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50–150 of human NDUFAB1; recognize that antibody reactivity may be influenced by protein conformation or post-translational modifications in native samples.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization protocols to preserve mitochondrial architecture and antigen accessibility.
• In ELISA applications, pair with a suitable detection antibody and validate using recombinant NDUFAB1 protein or cell lysates expressing the target.
• Confirmed cross-reactivity with mouse suggests potential utility in rodent model studies; however, confirm performance in the relevant tissue or cell system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This NDUFAB1 antibody aids research into mitochondrial metabolism, Complex I biology, and iron‑sulfur cluster biogenesis. Beyond individual reagents, we supply validated antibody pairs, optimized mono‑ and polyclonal antibodies, bulk ancillary reagents, and can source rare‑target raw materials to streamline your assay development workflow.