Anti-NDUFAB1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O14561

Core Product Specifications and Parameters

Biological Background: NDUFAB1 (Acyl carrier protein, mitochondrial) Function and Localization

• NDUFAB1 encodes the mitochondrial acyl carrier protein (ACP), also designated CI-SDAP and NADH-ubiquinone oxidoreductase 9.6 kDa subunit.
• It serves as the carrier of the growing fatty acid chain in mitochondrial fatty acid biosynthesis. Related references: PMID:27626371
• As an accessory subunit of respiratory chain Complex I, it is essential for NADH-to-ubiquinone electron transfer. Related references: PMID:27626371
• Within the core iron-sulfur cluster (ISC) assembly complex, NDUFAB1 together with LYRM4 regulates NFS1 cysteine desulfurase stability and activity, enabling [2Fe-2S] cluster synthesis on ISCU. Related references: PMID:31664822
• The protein is localized to the mitochondrion.
• Post-translational modifications include phosphopantetheinylation and acetylation, critical for its ACP functionality.
• Molecular weight of the mature protein is approximately 17 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 50–156) of human NDUFAB1, which shares high homology with mouse and rat orthologs, supporting the observed multi-species reactivity.
• For Western blot (WB), a ~17 kDa band is expected; use appropriate low molecular weight protein markers and consider running a reducing SDS-PAGE.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution; mitochondrial staining patterns should be validated in relevant tissues (e.g., heart, muscle).
• For ELISA, the antibody can be used as a capture or detection reagent; pairing with another clone may enhance assay sensitivity.
• Validate antibody performance in the specific sample matrix and application via appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material partner, connecting diagnostic developers with high-quality reagents. For targets like NDUFAB1 involved in mitochondrial function and metabolic research, CamelBio can source validated antibody pairs, bulk ancillary reagents, and rare raw materials to support assay development from concept to clinic.