Anti-NDUFAB1 Polyclonal Antibody for WB, ELISA - O14561

Core Product Specifications and Parameters

Biological Background: NDUFAB1 Function and Localization

• NDUFAB1 (mitochondrial acyl carrier protein) is a 17 kDa protein encoded by the NDUFAB1 gene, also known as CI-SDAP, ACP1, SDAP, and FASN2A.
• It functions as a carrier of the growing fatty acid chain in mitochondrial fatty acid biosynthesis. Related references: PMID:27626371
• As an accessory subunit of mitochondrial Complex I, NDUFAB1 participates in electron transfer from NADH to the respiratory chain. Related references: PMID:27626371
• NDUFAB1 is an accessory protein of the core iron-sulfur cluster (ISC) assembly complex, regulating NFS1 stability and cysteine desulfurase activity for [2Fe-2S] cluster formation. Related references: PMID:31664822
• The protein localizes exclusively to the mitochondrion, as indicated by its transit peptide and subcellular fractionation studies.
• Post-translational modifications include phosphopantetheinylation at the active site serine, acetylation, and phosphorylation, which may modulate its function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 50–156) of human NDUFAB1, conserved across human, mouse, and rat. For Western blot, expect a band near 17 kDa; validate detection in your sample system and consider mitochondrial enrichment for stronger signal.
• For ELISA, the immunizing peptide can serve as a positive control; assay conditions should be optimized empirically for each format.
• Optimal working dilutions must be determined by end users due to lot-to-lot variation and differences in protocols; document recommendations are not provided.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials and tailored technical support from concept to clinic. This NDUFAB1 polyclonal antibody exemplifies our ability to supply validated reagents for mitochondrial research and metabolic assay development. Beyond individual antibodies, we offer matched antigen controls, bulk ancillary reagents, and rare target sourcing—streamlining your procurement and accelerating project timelines.