Anti-NDUFA9 Polyclonal Antibody for WB and ELISA - Q16795

Core Product Specifications and Parameters

Biological Background: NDUFA9 Function and Localization

• NDUFA9 encodes an accessory subunit of mitochondrial respiratory chain Complex I (NADH dehydrogenase) that is essential for proper assembly of the holoenzyme but is not directly involved in catalysis. Related references: PMID:28671271
• As part of Complex I, NDUFA9 contributes to the transfer of electrons from NADH to ubiquinone, a critical step in oxidative phosphorylation.
• The protein localizes to the mitochondrial matrix, indicating its site of action within the organelle.
• According to UniProt keywords, NDUFA9 is a flavoprotein that binds FAD and is subject to acetylation.
• Disease variants in NDUFA9 are associated with primary mitochondrial disease, highlighting its clinical relevance.
• The protein is also known by alternative names such as CI-39kD, NDUFS2L, and SDR22E1.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1–270) of human NDUFA9; therefore, the antibody may preferentially recognize this portion of the protein.
• For Western blot, the expected band size is approximately 43 kDa; consider using whole-cell or mitochondrial-enriched lysates from human, mouse, or rat tissues.
• For ELISA, the antibody can be used as a capture or detection reagent; assay conditions should be optimized for the specific application.
• Cross-reactivity with mouse and rat has been specified; however, validation in the relevant species and sample type is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials and technical support from concept to clinic. This anti-NDUFA9 antibody supports mitochondrial and respiratory chain studies, and we can also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents tailored to your assay development needs. Whether you require rare target sourcing or large-scale production, CamelBio is your reliable partner in the IVD supply chain.