Anti-NDUFA8 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P51970

Core Product Specifications and Parameters

Biological Background: NDUFA8 Function and Localization

• NDUFA8, also known as Complex I-19kD (CI-19kD) or Complex I-PGIV (CI-PGIV), is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I).
• As a non-catalytic subunit, NDUFA8 is believed not to be directly involved in catalysis but is essential for the transfer of electrons from NADH to the respiratory chain, with ubiquinone as the immediate electron acceptor. Related references: PMID:27626371 PMID:32385911 PMID:33153867
• Subcellular localization studies indicate NDUFA8 resides in the mitochondrion inner membrane and intermembrane space.
• Keywords from UniProt include 3D-structure, direct protein sequencing, disease variant, disulfide bond, electron transport, membrane, mitochondrion, mitochondrion inner membrane, primary mitochondrial disease, proteomics identification, reference proteome, repeat, respiratory chain, and transport.
• The protein has a molecular weight of approximately 20 kDa and belongs to a family of Complex I accessory subunits critical for respiratory chain integrity.

Experimental Guidance and Technical Tips

• For Western blotting, a band near 20 kDa is anticipated based on the provided immunogen sequence (amino acids 33–172).
• The antibody is indicated for WB, IF/ICC, IP, and ELISA; consider optimizing dilution and detection conditions for your specific sample system.
• Cross-reactivity is reported with human, mouse, and rat samples; validate in your target species and tissue type before large-scale experiments.
• The immunogen is a recombinant protein; pre-immune serum or a blocking peptide (if available) may be used to confirm specificity.
• For mitochondrial targets, ensure proper sample preparation (e.g., mitochondrial enrichment) to enhance signal detection in IF/ICC.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated B2B partner for IVD raw materials, offering one-stop access to validated antibodies, antigen pairs, and ancillary reagents for diagnostic manufacturers and research laboratories. For targets like NDUFA8, a key mitochondrial Complex I subunit implicated in primary mitochondrial disease, we supply high-quality polyclonal antibodies and can support sourcing of rare raw materials, bulk orders, and customized development through our integrated technical network. Let CamelBio bridge your workflow from concept to clinic with reliable, research-ready solutions.