Anti-NDUFA7 Polyclonal Antibody (KO Validated) for WB & ELISA - O95182

Core Product Specifications and Parameters

Biological Background: NDUFA7 Function and Localization

• NDUFA7 (also known as CI-B14.5a or B14.5a) is an accessory subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase), the first enzyme of the respiratory chain.
• It is a non-catalytic subunit believed not to be directly involved in the electron transfer mechanism but contributes to the structural integrity and assembly of Complex I.
• Complex I catalyzes the transfer of electrons from NADH to ubiquinone, coupling this process with proton translocation across the inner mitochondrial membrane.
• Localized to the mitochondrial inner membrane, NDUFA7 is an integral component of the membrane arm of Complex I.
• Post-translational modifications include acetylation and phosphorylation, which may regulate Complex I activity or assembly.
• Keywords associated with NDUFA7 include 3D-structure, electron transport, membrane, mitochondrion, respiratory chain, and transport.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length NDUFA7 sequence (aa 1–113); therefore, the antibody is expected to detect the native protein in mitochondrial extracts.
• For Western blot, the predicted molecular weight is approximately 13 kDa (product specification) or 12.5 kDa (UniProt canonical sequence); validate with positive controls such as human, mouse, or rat mitochondrial fractions.
• This polyclonal antibody has been KO validated, indicating specificity; however, we recommend confirming expression levels in your experimental system, especially if using non-listed species or tissues.
• For ELISA applications, consider pairing with a monoclonal detection antibody to improve assay sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a seamless procurement experience for IVD raw materials, including this high-quality anti-NDUFA7 polyclonal antibody. Whether you need validated antibody pairs for respiratory chain targets, bulk ancillary reagents, or custom sourcing of rare mitochondrial markers, our integrated supply chain supports your diagnostic development from early research to commercial kits.