Anti-NDUFA6 Rabbit Polyclonal Antibody for WB and ELISA - P56556

Core Product Specifications and Parameters

Biological Background: NDUFA6 Function and Localization

• NDUFA6 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6), also known as Complex I-B14, LYRM6, or NADH-ubiquinone oxidoreductase B14 subunit, is an accessory subunit of the mitochondrial respiratory chain Complex I.
• It is believed not to be directly involved in catalysis but is required for proper Complex I assembly. Related references: PMID:30245030
• Complex I mediates the transfer of electrons from NADH to ubiquinone, and NDUFA6 contributes to the structural integrity of this multimeric enzyme.
• Subcellular localization: Mitochondrion inner membrane.
• It belongs to the LYR motif-containing protein family and is subject to phosphorylation.
• Mutations in NDUFA6 are associated with mitochondrial complex I deficiency (MC1DN33), a primary mitochondrial disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 35-154 of human NDUFA6, and the antibody may detect endogenous NDUFA6 in human, mouse, and rat samples.
• For Western blot, a band of approximately 15 kDa is expected; consider validating specificity using relevant positive and negative controls.
• In ELISA applications, performance may vary with different antigen presentation formats; optimize the coating concentration and detection system.
• Cross-reactivity with other species or isoforms should be assessed in the experimental system of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and research laboratories, providing validated antibody pairs, optimized recombinant antibodies, and bulk ancillary reagents for mitochondrial and respiratory chain research. For your NDUFA6 and Complex I projects, we offer efficient raw-material sourcing and supply solutions, bridging your needs from concept to clinic with scientific support and reliable logistics.