Anti-NDUFA5 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16718

Core Product Specifications and Parameters

Biological Background: NDUFA5 Function and Localization

• NDUFA5, also known as Complex I subunit B13 or CI-13kD-B, is an accessory subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase).
• It is not directly involved in catalysis but is essential for Complex I stability and function, facilitating electron transfer from NADH to ubiquinone in the respiratory chain.
• Encoded by the NDUFA5 gene (GeneID: 4698) in humans, it localizes to the mitochondrial inner membrane as part of the peripheral arm of Complex I.
• NDUFA5 is ubiquitously expressed, with the highest levels detected in heart, skeletal muscle, and brain, reflecting its critical role in tissues with high energy demands.
• The protein undergoes N-terminal acetylation and is subject to alternative splicing, generating multiple isoforms.
• Structural studies (as part of Complex I) reveal its position in the N-module, contributing to the architecture of the NADH-oxidizing domain.

Experimental Guidance and Technical Tips

• The immunogen (recombinant protein, aa 1-116 of human NDUFA5) targets the highly conserved N-terminus, supporting cross-reactivity with mouse and rat. Validate in relevant species.
• For Western blotting, expect a band around 13 kDa; use lysates from heart, skeletal muscle, or brain for optimal detection.
• In IHC-P and IF/ICC, ensure mitochondrial staining patterns through appropriate fixation, antigen retrieval, and permeabilization.
• ELISA can be optimized using the recombinant immunogen as a positive control in direct or indirect formats.
• As a polyclonal antibody, verify lot-to-lot consistency for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material partner, providing diagnostic manufacturers and labs with a comprehensive portfolio of antibodies, antigens, and ancillary reagents. This anti-NDUFA5 antibody supports research into mitochondrial Complex I deficiencies, metabolic disorders, and neurodegenerative diseases. We specialize in sourcing rare target reagents and offering technical consulting to streamline your assay development from concept to clinic.