Anti-NDUFA5 Polyclonal Antibody for WB, ELISA - Q16718

Core Product Specifications and Parameters

Biological Background: NDUFA5 Function and Localization

• NDUFA5, also known as Complex I subunit B13 or CI-13kD-B, is an accessory subunit of the mitochondrial NADH dehydrogenase (Complex I) and is not directly involved in catalysis.
• Complex I catalyzes the transfer of electrons from NADH to ubiquinone in the respiratory chain, and NDUFA5 contributes to the structural integrity of the complex.
• The protein localizes to the mitochondrial inner membrane.
• It is ubiquitously expressed, with highest levels observed in heart, skeletal muscle, and brain.
• Post-translational modifications include acetylation, and the gene undergoes alternative splicing.
• Compatible detection of NDUFA5 is relevant for studies on mitochondrial function and disorders affecting the respiratory chain.

Experimental Guidance and Technical Tips

• Due to the immunogen design covering the N-terminal region (aa1-116), the antibody likely recognizes the native protein across listed species. Validate reactivity in your specific experimental system.
• For WB, consider using mitochondrial-enriched lysates or whole cell lysates, and include appropriate controls (e.g., siRNA knockdown or tissues with known expression levels).
• For ELISA, the antibody may be used for capture or detection; optimal working dilutions should be determined experimentally.
• Since Complex I is a large multi-subunit complex, mild lysis conditions may be required to preserve native protein interactions if studying the intact complex.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers by providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This anti-NDUFA5 antibody is ideal for mitochondrial respiratory chain research and assay development. Let CamelBio streamline your IVD raw material procurement from concept to clinic.