Anti-NDUFA4L2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9NRX3

Core Product Specifications and Parameters

Biological Background: NDUFA4L2 Function and Localization

• NDUFA4L2, also designated COXFA4L2 or NUOMS, is a mitochondrial protein encoded by the COXFA4L2 gene and belongs to the NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 4-like family.
• Under hypoxic conditions, NDUFA4L2 downregulates mitochondrial respiratory chain complex I activity, thereby reducing oxygen consumption and reactive oxygen species (ROS) production, and promoting a metabolic shift toward glycolysis to facilitate cellular adaptation to low-oxygen environments. Related references: PMID:22100406
• The protein is localized to the mitochondrial inner membrane, a topology consistent with its role in modulating oxidative phosphorylation.
• With a molecular weight of approximately 10 kDa, NDUFA4L2 contains predicted transmembrane helices, indicating its integration into the mitochondrial membrane.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–87) of human NDUFA4L2; consider verifying that the antibody recognizes the endogenous full-length protein in your model system.
• For WB, use appropriate positive and negative controls (e.g., hypoxia-treated vs. normoxic cells) and optimize loading amounts to detect the 10 kDa target clearly.
• In IHC-P and IF/ICC experiments, antigen retrieval steps and proper fixation protocols should be standardized, as membrane proteins may exhibit variable accessibility.
• Cross-reactivity with mouse and rat is listed, but confirm reactivity with your specific tissues or cell lines, as epitope conservation should be verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. As a supplier of this NDUFA4L2 antibody for hypoxia and mitochondrial metabolism studies, CamelBio supports your workflow from concept to clinic with sourcing and technical consulting for rare-target and custom projects.