Anti-NDUFA13/GRIM19 Rabbit Monoclonal Antibody for WB, ELISA - Q9P0J0

Core Product Specifications and Parameters

Biological Background: NDUFA13/GRIM19 Function and Localization

• NDUFA13 (also known as GRIM-19, B16.6, CGI-39) is a nuclear-encoded accessory subunit of mitochondrial Complex I, essential for complex assembly but not believed to participate directly in catalytic electron transfer. Related references: PMID:27626371
• It functions in the transfer of electrons from NADH to ubiquinone as part of the respiratory chain, contributing to oxidative phosphorylation.
• NDUFA13 is a key mediator of interferon/all-trans-retinoic acid (IFN/RA)-induced cell death, and its pro-apoptotic activity is inhibited by viral IRF1, highlighting a role in host-virus interactions.
• It may regulate intestinal epithelial cell responses to microbes by participating in CARD15-mediated innate mucosal responses, with implications in inflammatory bowel diseases such as Crohn disease and ulcerative colitis. Related references: PMID:15753091
• NDUFA13 also suppresses STAT3 target gene transactivation, modulating cell survival and proliferation pathways.
• Subcellularly, it localizes predominantly to the mitochondrial inner membrane, but has been detected in the nucleus, suggesting possible dual roles.
• Widely expressed, with highest levels in heart, skeletal muscle, liver, kidney, and placenta; expression is down-regulated in inflamed intestinal mucosa.
• Post-translational modifications include acetylation, and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal 144 amino acids; consider that this region may be conserved across isoforms for consistent detection.
• For Western blot, the theoretical molecular weight is ~17 kDa; however, observe actual migration patterns in your sample system as post-translational modifications or complex association may cause shifts.
• In ELISA, confirm optimal antibody dilution and assay format (direct or capture) using recombinant NDUFA13 or relevant cell lysates.
• Given the reported human cross-reactivity, validate species specificity if used in non-human models.
• The antibody’s suitability for ChIP or IF has not been characterized; if needed, perform pilot tests with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials and tailored sourcing solutions. This NDUFA13/GRIM19 monoclonal antibody supports mitochondrial biology, apoptosis, and innate immunity research, and we are ready to assist with validated antibody pairs, bulk ancillary reagents, and rare target raw-material procurement. From concept to clinic, count on CamelBio as your reliable sourcing partner.