Anti-NDUFA12 Rabbit Polyclonal Antibody for WB - Q9UI09

Core Product Specifications and Parameters

Biological Background: NDUFA12 Function and Localization

• NDUFA12 is an accessory subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase). It is not directly involved in catalysis but contributes to the structural integrity of the enzyme complex.
• The protein is also known as 13 kDa differentiation-associated protein, Complex I-B17.2, and NADH-ubiquinone oxidoreductase subunit B17.2. It is encoded by the NDUFA12 gene (formerly DAP13), with alternative splicing producing multiple isoforms.
• Complex I functions in the transfer of electrons from NADH to ubiquinone, a critical step in oxidative phosphorylation. NDUFA12 is one of numerous accessory subunits that stabilize and regulate the core catalytic subunits.
• It localizes to the mitochondrial inner membrane, consistent with its role in the respiratory chain.
• Post-translational modifications include acetylation, and mutations in NDUFA12 have been associated with primary mitochondrial disease (e.g., MC1DN23), highlighting its clinical significance.
• The protein has a predicted molecular weight of ~17.1 kDa (UniProt) and a product-observed weight of 17 kDa, typical for the mature form.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1-145 of full-length human NDUFA12, covering the entire protein. The resulting rabbit polyclonal antibody is expected to recognize multiple epitopes, offering robust detection in denaturing applications like WB.
• For WB, consider using reducing conditions and standard whole cell or tissue lysates from human, mouse, or rat. Mitochondrial enrichment may improve sensitivity due to the mitochondrial membrane localization of the target.
• ELISA applications can be explored; however, optimal coating and detection conditions should be empirically determined.
• Cross-reactivity with mouse and rat has been indicated, making this antibody suitable for multi-species studies, but validation in each experimental system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for in vitro diagnostics, offering a curated portfolio of high-quality raw materials including validated antibodies, antigen pairs, and ancillary reagents. This NDUFA12 rabbit polyclonal antibody is part of our mitochondrial research collection, designed to support the development of diagnostic assays targeting Complex I deficiencies. Through our one-stop platform, we facilitate access to rare-target antibodies and bulk custom production to streamline your IVD workflow from concept to clinic.