Anti-NDUFA10 (CI-42kD) Rabbit Polyclonal Antibody for WB and ELISA - O95299

Core Product Specifications and Parameters

Biological Background: NDUFA10 (CI-42kD) Function and Localization

• NDUFA10, also known as CI-42kD (Complex I-42kD), is the 42 kDa subunit of the mitochondrial NADH dehydrogenase (Complex I), encoded by the NDUFA10 gene.
• It functions as an accessory subunit of Complex I, not directly involved in catalysis, but essential for the assembly, stability, or regulation of the complex. Complex I mediates electron transfer from NADH to ubiquinone in the respiratory chain.
• Localized to the mitochondrial matrix, NDUFA10 is imported into mitochondria and associates with the inner membrane as part of the peripheral arm of Complex I.
• The protein contains FAD and is classified as a flavoprotein, involved in electron transport and respiratory chain processes.
• Post-translational modifications include phosphorylation, and the gene undergoes alternative splicing, potentially generating isoforms with functional diversity.
• Mutations in NDUFA10 are associated with mitochondrial complex I deficiency, which leads to a spectrum of primary mitochondrial diseases, including MC1DN22 (mitochondrial complex I deficiency, nuclear type 22).
• Structurally, NDUFA10 has been studied by 3D-structural analysis, providing insights into Complex I architecture.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 36-300 of human NDUFA10, excluding the mitochondrial transit peptide (a.a. 1-35). This design likely targets the mature, processed form of the protein.
• For Western blotting, the expected molecular weight is ~41 kDa. Consider using mitochondrial-enriched fractions to enhance detection.
• ELISA applications may benefit from using recombinant NDUFA10 protein or cell lysates; validate assay conditions in your specific sample matrix.
• When working with rodent samples, confirm reactivity under your experimental conditions, as cross-reactivity is reported for mouse and rat.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with reliable access to high-quality IVD raw materials. This anti-NDUFA10 antibody is part of a comprehensive portfolio targeting mitochondrial and metabolic disease markers. For your Complex I and respiratory chain projects, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents—streamlining your sourcing from concept to clinic.